| 產(chǎn)品編號 | bsm-60663R |
| 英文名稱 | Histone H2A (Acetyl-Lys9) Recombinant Rabbit mAb |
| 中文名稱 | 乙?；M蛋白H2A兔單克隆抗體 |
| 別 名 | H2AC4 | Histone H2A (ac-K9); ac-Histone H2A; acetyl-Histone H2A; H2AK9Ac; ac-H2A; H2A.1; H2A.2; H2A/a; H2AC4; H2AFA; HIST1H2AE; H2AB; HIST2H2AB; H2A/m; H2AC8; H2AFM; HIST1H2AB; H2A; H2A-GL101; H2A/q; H2AFQ; HIST2H2AC; 1190022L06; H2A1B_HUMAN; Histone H2A. |
| 產(chǎn)品類型 | 乙酰化抗體 重組兔單抗 |
| 研究領(lǐng)域 | 信號轉(zhuǎn)導(dǎo) 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | R5F7 |
| 交叉反應(yīng) | Human,Mouse |
| 產(chǎn)品應(yīng)用 | WB=1:500-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 14 kDa |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞外基質(zhì) |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Histone H2A (Acetyl-Lys9) |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] Subcellular Location: Cytoplasm; cytoskeleton. Tissue Specificity: Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues. SWISS: Q99878 Gene ID: 8338 Database links:
Entrez Gene: 3012 Human Entrez Gene: 317772 Human Entrez Gene: 8335 Human Entrez Gene: 8337 Human Entrez Gene: 8338 Human Entrez Gene: 319166 Mouse Omim: 142720 Human Omim: 602786 Human Omim: 602797 Human SwissProt: P04908 Human SwissProt: P28001 Human SwissProt: P35065 Human SwissProt: Q93077 Human SwissProt: Q99878 Human SwissProt: P10812 Mouse SwissProt: P22752 Mouse Unigene: 121017 Human Unigene: 248174 Human Unigene: 408067 Human Unigene: 417332 Human Unigene: 434195 Human Unigene: 261665 Mouse
|
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 4℃ overnight
Secondary Ab: (-): NIH/3T3, (+): NIH/3T3+ trichostain
A (1μM, 18 hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 14 KDa
Observed MW: 14 KDa
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 4℃ overnight
Secondary Ab: (-): MCF-7, (+): MCF-7+ sodium
butyrate (50mM, 24hr) + trichostain A (500ng/ml, 4 hr)
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 14 KDa
Observed MW: 14 KDa
|
