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產(chǎn)品編號(hào)	bs-0061R
英文名稱	beta-Actin Rabbit pAb, Loading Control
中文名稱	β-肌動(dòng)蛋白/β-Actin（內(nèi)參）抗體
別名	ACTB_HUMAN; Actin, cytoplasmic 1; ACTB; EC:3.6.4.- ; Beta actin; Actin, cytoplasmic 1, N-terminally processed; actin beta; BNS; CSMH; DDS1; THC8; BKRNS; BRWS1; PS1TP5BP1; Actin cytoplasmic 1; β actin; β-actin;
	Specific References (1140) \| bs-0061R has been referenced in 1140 publications. [IF=3.192] WB ; Pig. PubMed:32888967 [IF=4.087] WB ; Rat. PubMed:33300653 [IF=20.042] Xiang-Qian Gao. et al. The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N 6 -methyladenosine methylation of Parp10 mRNA. Nat Cell Biol. 2020 Oct;22(11):1319-1331 WB ; Mouse. PubMed:33020597 [IF=19.227] Xin Zhou. et al. ACSL4 promotes microglia-mediated neuroinflammation by regulating lipid metabolism and VGLL4 expression. BRAIN BEHAV IMMUN. 2023 Mar;109:331 WB ; Mouse. PubMed:36791893 [IF=18.027] Nguyen Thi Nguyen. et al. Amplified Fenton-Based Oxidative Stress Utilizing Ultraviolet Upconversion Luminescence-Fueled Nanoreactors for Apoptosis-Strengthened Ferroptosis Anticancer Therapy. ACS NANO. 2022;XXXX(XXX):XXX-XXX WB ; Mouse. PubMed:36579941 [IF=16.744] Rui Liu. et al. Engineered stem cell biomimetic liposomes carrying levamisole for macrophage immunity reconstruction in leukemia therapy. CHEM ENG J. 2022 Nov;447:137582 WB ; Rat. PubMed:10.1016/j.cej.2022.137582 [IF=15.84] Xiaolie He. et al. MgFe‐LDH Nanoparticles: A Promising Leukemia Inhibitory Factor Replacement for Self‐Renewal and Pluripotency Maintenance in Cultured Mouse Embryonic Stem Cells. 2021 Feb 25 WB ; Mouse. PubMed:10.1002/advs.202003535 [IF=15.304] Yao Lei. et al. Phytochemical natural killer cells reprogram tumor microenvironment for potent immunotherapy of solid tumors. BIOMATERIALS. 2022 Jun;:121635 WB ; Mouse. PubMed:10.1016/j.biomaterials.2022.121635 [IF=14.976] Qinyu Ma. et al. Small extracellular vesicles deliver osteolytic effectors and mediate cancer‐induced osteolysis in bone metastatic niche. J Extracell Vesicles. 2021 Feb;10(4):e12068 WB ; Mouse. PubMed:33659051 [IF=14.903] Shuai Xiong. et al. Glutamate-releasing BEST1 channel is a new target for neuroprotection against ischemic stroke with wide time window. ACTA PHARM SIN B. 2023 May;: WB ; Mouse. PubMed:10.1016/j.apsb.2023.05.012 [IF=14.26] Mi Bai. et al. LONP1 targets HMGCS2 to protect mitochondrial function and attenuate chronic kidney disease. EMBO MOL MED. 2023 Jan 11 WB ; Mouse. PubMed:36629048 [IF=14.026] Congcong Chen. et al. Radix Paeoniae Alba attenuates Radix Bupleuri-induced hepatotoxicity by modulating gut microbiota to alleviate the inhibition of saikosaponins on glutathione synthetase. J PHARM ANAL. 2023 Apr;: WB ; Rat. PubMed:10.1016/j.jpha.2023.04.016 [IF=13.273] Danmin Lin. et al. Brain-targeted gene delivery of ZnO quantum dots nanoplatform for the treatment of Parkinson disease. Chem Eng J. 2022 Feb;429:132210 WB ; Human. PubMed:10.1016/j.cej.2021.132210 [IF=13.273] Long Zhao. et al. Juglone-loaded metal-organic frameworks for H2O2 self-modulating enhancing chemodynamic therapy against prostate cancer. Chem Eng J. 2022 Feb;430:133057 WB ; Human. PubMed:10.1016/j.cej.2021.133057 [IF=13.273] Xinkun Shen. et al. Improvement of aqueous stability and anti-osteoporosis properties of Zn-MOF coatings on titanium implants by hydrophobic raloxifene. Chem Eng J. 2021 Oct;:133094 WB ; Mouse. PubMed:10.1016/j.cej.2021.133094 [IF=12.336] Baikai Ma. et al. Pigment epithelium-derived factor (PEDF) plays anti-inflammatory roles in the pathogenesis of dry eye disease. Ocul Surf. 2021 Apr;20:70 WB ; Human. PubMed:10.1016/j.jtos.2020.12.007 [IF=11.556] Wen-juan Jiang. et al. Tubular epithelial cell-to-macrophage communication forms a negative feedback loop via extracellular vesicle transfer to promote renal inflammation and apoptosis in diabetic nephropathy. Theranostics. 2022; 12(1): 324–339 WB ; Mouse,Human. PubMed:34987648 [IF=11.48] Lan, Feng, et al. "Forkhead box protein 3 in human nasal polyp regulatory T cells is regulated by the protein suppressor of cytokine signaling 3." Journal of Allergy and Clinical Immunology 132.6 (2013): 1314-1321. WB ; Human. PubMed:23910692 [IF=11.33] Leclercq, Sophie, et al. "Low-dose penicillin in early life induces long-term changes in murine gut microbiota, brain cytokines and behavior." Nature Communications 8 (2017): 15062. WB ; Mouse. PubMed:28375200 [IF=11.161] Zhipeng Jiang. et al. EIF4A3-induced circ_0084615 contributes to the progression of colorectal cancer via miR-599/ONECUT2 pathway. J Exp Clin Canc Res. 2021 Dec;40(1):1-15 WB ; Human. PubMed:34253241 [IF=10.787] Xiaozhen Dai. et al. Nrf2 transcriptional upregulation of IDH2 to tune mitochondrial dynamics and rescue angiogenic function of diabetic EPCs. REDOX BIOL. 2022 Oct;56:102449 WB ; Mouse. PubMed:36063728 [IF=10.787] Yi Zhao. et al. Ferroptosis is critical for phthalates driving the blood-testis barrier dysfunction via targeting transferrin receptor. REDOX BIOL. 2023 Feb;59:102584 WB ; Mouse. PubMed:36580806 [IF=10.753] Fangwei Yang. et al. Diethyl phosphate disrupts hypothalamus-pituitary-adrenal axis endocrine hormones via nuclear receptors GR and Nur77: Integration of evidences from in vivo, in vitro and in silico approaches. SCI TOTAL ENVIRON. 2022 Oct;844:157015 WB ; Rat, Human. PubMed:35777568 [IF=10.753] Ya-Nan Gao. et al. Aflatoxin M1 and ochratoxin A induce a competitive endogenous RNA regulatory network of intestinal immunosuppression by whole-transcriptome analysis. SCI TOTAL ENVIRON. 2022 Sep;:158777 WB ; Human. PubMed:36115400 [IF=10.75] Hu Yifang. et al. S100 Calcium Binding Protein A16 Promotes Cell Proliferation by triggering LATS1 ubiquitin degradation mediated by CUL4A ligase to inhibit Hippo pathway in Glioma development. INT J BIOL SCI. 2023 Apr;19(7):2034-2052 WB ; Human. PubMed:37151881 [IF=10.383] Zhen Xu. et al. Green Biosynthesis of Silver Nanoparticles Using Aqueous Extracts of Ageratum Conyzoides and Their Anti-Inflammatory Effects. ACS APPL MATER INTER. 2023;XXXX(XXX):XXX-XXX WB ; Mouse,Human. PubMed:36881383 [IF=10.19] Xianqiang Li. et al. Menthol nanoliposomes enhanced anti-tumor immunotherapy by increasing lymph node homing of dendritic cell vaccines. CLIN IMMUNOL. 2022 Sep;:109119 WB ; Mouse. PubMed:36109005 [IF=10.171] Ping Pang. et al. The circular RNA circHelz enhances cardiac fibrosis by facilitating the nuclear translocation of YAP1. TRANSL RES. 2023 Feb;: WB ; Mouse. PubMed:36775059 [IF=9.933] Xingyi Xu. et al. A Honeycomb-Like Bismuth/Manganese Oxide Nanoparticle with Mutual Reinforcement of Internal and External Response for Triple-Negative Breast Cancer Targeted Therapy. 2021 Jul 23 WB ; Human. PubMed:34297897 [IF=9.918] Jie Hao. et al. Multifunctional miR181a nanoparticles promote highly efficient radiotherapy for rectal cancer. MATER TODAY ADV. 2022 Dec;16:100317 WB ; Mouse. PubMed:10.1016/j.mtadv.2022.100317
產(chǎn)品類型	內(nèi)參抗體
研究領(lǐng)域	腫瘤細(xì)胞生物信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞骨架
抗體來源	Rabbit
克隆類型	Polyclonal
克隆號(hào)
交叉反應(yīng)	Human,Mouse,Rat,Hamster (predicted: Rabbit,Pig,Sheep,Chicken,Dog,Cat,GuineaPig,Fish,Bee)
產(chǎn)品應(yīng)用	WB=1:5000-50000,IHC-P=1:200-1000,IHC-F=1:200-1000,IF=1:200-1000,Flow-Cyt=1μg/Test,ICC/IF=1:100-500 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
理論分子量	42 kDa
檢測分子量
細(xì)胞定位	細(xì)胞漿
性狀	Liquid
濃度	1mg/ml
免疫原	Synthetic MAP peptide derived from human beta-Actin: 1-200/375
亞型	IgG
純化方法	affinity purified by Protein A
緩沖液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng)	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
產(chǎn)品介紹	Loading Control This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]. Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Subunit: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Subcellular Location: Cytoplasm. cytoskeleton. Tissue Specificity: Ubiquitously expressed in all eukaryotic cells. Post-translational modifications: ISGylated. Oxidation of Met-44 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced. DISEASE: Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed. Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent. Similarity: Belongs to the actin family. SWISS: P60709 Gene ID: 60 Database links: Entrez Gene: 396526 Chicken Entrez Gene: 60 Human Entrez Gene: 11461 Mouse Entrez Gene: 100009272 Rabbit Entrez Gene: 81822 Rat Omim: 102630 Human SwissProt: P60706 Chicken SwissProt: P60712 Cow SwissProt: P60708 Horse SwissProt: P60709 Human SwissProt: P60710 Mouse SwissProt: P29751 Rabbit SwissProt: P60711 Rat SwissProt: P60713 Sheep Unigene: 520640 Human Unigene: 708120 Human Unigene: 727576 Human Unigene: 328431 Mouse Unigene: 391967 Mouse Unigene: 94978 Rat 內(nèi)參抗體 β-Actin是橫紋肌肌纖維中的一種主要蛋白質(zhì)成分，也是肌肉細(xì)絲及細(xì)胞骨架微絲的主要成分。具有收縮功能，分布廣泛，具有高度保守性，在細(xì)胞中的表達(dá)相對穩(wěn)定，因此常被用作校正系統(tǒng)的內(nèi)參。β-Actin分子量為42 kDa，此抗體主要用于標(biāo)記平滑肌及其來源的腫瘤。我公司開發(fā)的β-Actin抗體已被國內(nèi)外廣大科研工作者使用，被稱為質(zhì)量信得過產(chǎn)品.
產(chǎn)品圖片	Sample: SH-SY5Y (Human) Lysate at 40 ug Primary: Anti-beta-Actin (bs-0061R) at 1/2000~1/20000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Sample:Embryo Cerebrum (Mouse) Lysate at 40 ugDu145 (Human) Lysate at 40 ugSW480 (Human) Cell Lysate at 40 ugU87MG (Human) Lysate at 40 ugU251 (Human) Lysate at 40 ugA673 (Human) Lysate at 40 ugLovo (Human) Lysate at 40 ug293FT (Human) Lysate at 40 ugJEG-3 (Human) Lysate at 40 ugRSC96 (Rat) Cell Lysate at 40 ugMCF-7 (Human) Cell Lysate at 40 ugHepG2 (Human) Lysate at 40 ugA431 (Human) Lysate at 40 ugPrimary: Anti-beta-Actin (bs-0061R) at 1/2000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 42 kDObserved band size: 42 kD Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Small Intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Rat Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Mouse Uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Rat Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Paraformaldehyde-fixed, paraffin embedded Mouse Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with beta-Actin Polyclonal Antibody, Unconjugated (bs-0061R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining. Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (beta-Actin) polyclonal Antibody, Unconjugated (bs-0061R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG-FITC antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (beta-Actin) polyclonal Antibody, Unconjugated (bs-0061R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control: NIH/3T3. Primary Antibody (green line): Rabbit Anti-beta-Actin (Loading Control) antibody (bs-0061R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

	免疫組化常用試劑
	免疫印跡常用試劑
	各種標(biāo)記二抗

	1、抗體溶解方法
	2、抗體修復(fù)方式
	3、常用試劑的配制
	4、免疫組化操作步驟
	5、免疫組化問題解答
	6、Western Blotting 操作步驟
	7、Western Blotting 問題解答
	8、關(guān)于肽鏈的設(shè)計(jì)
	9、多肽的溶解與保存
	10、酶標(biāo)抗體效價(jià)測定程序

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