| 產品編號 | bs-18767R |
| 英文名稱 | MED28 Rabbit pAb |
| 中文名稱 | MED28蛋白抗體 |
| 別 名 | 1500003D12Rik; EG1; magicin; FKSG20; MED28_HUMAN; MED28; Endothelial-derived protein 1; Mediator complex subunit 28; Merlin and Grb2-interacting cytoskeletal protein (Magicin); Tumor angiogenesis marker EG-1; MED28_MOUSE; |
| 研究領域 | 細胞生物 轉錄調節(jié)因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse (predicted: Human,Rat,Rabbit,Pig,Sheep,Cow,Zebrafish,Chicken,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 20 kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MED28: 1-100/178 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Med28 is a 178 amino acid subunit of the Mediator complex that localizes to both nucleus and cytoplasm. Widely expressed with highest expression in vascular tissues such as placenta, testis and liver, Med28 participates in the regulation of Pol II-mediated gene expression and is thought to play a key role in signal regulation and cell proliferation. It is suggested that Med28 functions as a repressor of SMC (smooth muscle cell) differentiation and may be associated with abnormalities in SMC growth and differentiation, including atherosclerosis, asthma, hypertension and smooth muscle tumors. Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways. Subcellular Location: Nucleus. Cytoplasm. Membrane. According to PubMed:15467741, it is cytoplasmic and mainly membrane-associated. Tissue Specificity: Widely expressed. Highly expressed in vascular tissues such as placenta, testis and liver. Similarity: Belongs to the Mediator complex subunit 28 family. SWISS: Q9H204 Gene ID: 80306 Database links: Entrez Gene: 80306 Human Entrez Gene: 66999 Mouse Omim: 610311 Human SwissProt: Q9H204 Human SwissProt: Q920D3 Mouse Unigene: 434075 Human Unigene: 644788 Human Unigene: 283045 Mouse |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MED28) Polyclonal Antibody, Unconjugated (bs-18767R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MED28) Polyclonal Antibody, Unconjugated (bs-18767R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
