| 產(chǎn)品編號 | bs-17403R |
| 英文名稱 | HIPK1 Rabbit pAb |
| 中文名稱 | 核小體相關(guān)激酶HIPK1抗體 |
| 別 名 | Myak; Nbak2; 1110062K04Rik; HIPK1_HUMAN; HIPK1; Nuclear body-associated kinase 2; 2.7.11.1; KIAA0630; HIPK1_MOUSE; Protein kinase Myak; HIPK1_RAT; |
| 研究領(lǐng)域 | 細胞生物 信號轉(zhuǎn)導(dǎo) 激酶和磷酸酶 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Dog,Horse, Macaque Monkey) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 131 kDa |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HIPK1: 1-100/1210 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene belongs to the Ser/Thr family of protein kinases and HIPK subfamily. It phosphorylates homeodomain transcription factors and may also function as a co-repressor for homeodomain transcription factors. Alternative splicing results in four transcript variants encoding four distinct isoforms. [provided by RefSeq, Jul 2008] Function: May play a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. May be involved in malignant squamous cell tumor formation. Subcellular Location: Nucleus. Cytoplasm. Predominantly nuclear. Tissue Specificity: Ubiquitously expressed with highest levels in skeletal muscle and heart. Overexpressed in breast cancer cell lines. Post-translational modifications: Autophosphorylated. Phosphorylated and activated by JNK1. Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily. Contains 1 protein kinase domain. SWISS: Q86Z02 Gene ID: 204851 Database links: Entrez Gene: 204851 Human Entrez Gene: 15257 Mouse Omim: 608003 Human SwissProt: Q86Z02 Human SwissProt: O88904 Mouse Unigene: 532363 Human Unigene: 20827 Mouse Unigene: 4151 Rat |
| 產(chǎn)品圖片 |
Sample:
Mcf-7(Human) Cell Lysate at 40 ug
Primary: Anti-HIPK1(bs-17403R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 131 kD
Observed band size: 131kD
Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Rat Testis tissue lysates
Lane 3: Human HeLa cell lysates
Lane 4: Human 293T cell lysates
Primary: Anti-HIPK1 (bs-17403R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 131 kDa
Observed band size: 130 kDa
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HIPK1) Polyclonal Antibody, Unconjugated (bs-17403R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
