| 產(chǎn)品編號 | bs-13486R |
| 英文名稱 | GOLGA1 Rabbit pAb |
| 中文名稱 | 高爾基體蛋白97抗體 |
| 別 名 | GOGA1_HUMAN; Golgin subfamily A member 1; Golgin 97; Golgin-97; Golgin97; |
| 研究領(lǐng)域 | 細胞生物 細胞類型標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 88 kDa |
| 檢測分子量 | 90-100 |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GOLGA1: 51-150/767 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The GRIP family member, golgin 97, is a trans-Golgi network peripheral membrane protein with an extensive coiled-coil structure (67% alpha-helical content) and a C-terminal GRIP domain. Golgin 97 localizes exclusively on the cytoplasmic face of the Golgi and can form homodimers. Binding of golgin 97 to the Golgi membrane is mediated by the G protein family member, Arl1. Golgin 97 acts as an essential player to the cell in the form of a tethering molecule associating with tubulovesicular carriers during the trafficking from the trans-Golgi network to the recycling endosome and/or early endosome. During poxvirus infection, golgin 97 accumulates at the site of viral replication and is incorporated into virions. It associates with the insoluble fraction of the virus core protein, playing a significant role in virus replication and maturation of the virus membrane and core protein. Golgin 97 takes on a rod-like shape and, although it seemingly lacks a transmembrane domain, it protrudes from the surface of the virion envelope. Function: Probably involved in maintaining Golgi structure. Subunit: Binds RAB6A. Subcellular Location: Golgi apparatus, Golgi stack membrane; Peripheral membrane protein. Similarity: Contains 1 GRIP domain. SWISS: Q92805 Gene ID: 2800 Database links: Entrez Gene: 2800 Human Entrez Gene: 76899 Mouse SwissProt: Q92805 Human SwissProt: Q9CW79 Mouse |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Cerebrum tissue lysates
Lane 3: Rat Testis tissue lysates
Lane 4: Rat Cerebrum tissue lysates
Lane 5: Human HepG2 cell lysates
Lane 6: Human MDA-MB-231 cell lysates
Lane 7: Human HeLa cell lysates
Lane 8: Human MCF-7 cell lysates
Lane 9: Human A431 cell lysates
Primary: Anti-GOLGA1 (bs-13486R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 88 kDa
Observed band size: 100 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
