| 產(chǎn)品編號(hào) | bs-3917R |
| 英文名稱 | WIF1 Rabbit pAb |
| 中文名稱 | Wnt信號(hào)抑制因子1抗體 |
| 別 名 | WIF-1; WIF1_HUMAN; WIF1; WIF1_MOUSE; WIF1_RAT; |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | (predicted: Human,Mouse,Rat,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 39 kDa |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human WIF1: 51-150/379 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The Wnt genes are a group of conserved, Cysteine-rich, secreted glycoproteins that are required for numerous developmental processes including embryogenesis, asymmetric cell division and central nervous system (CNS) patterning. Wnt association with the transmembrane spanning receptor frizzled activates dishevelled, which downregulates glycogen synthase kinase (GSK) through serine phosphorylation. Reduced levels of active GSK causes accumulation of b-catenin and subsequent regulation of developmentally significant Wnt target genes. Wnt antagonists such as dickkopf (Dkk), frizzled-related protein (sFRP) and Wnt inhibitory factor-1 (WIF-1), are necessary to ensure normal spatial and temporal patterns of Wnt activity during developmental processes. WIF-1 is a 379-amino acid secreted protein that contains an N-terminal signal sequence, a 150-amino acid WIF domain, five epidermal growth factor-like repeats and a 45-amino acid C-terminal hydrophilic domain. Subcellular Location: Secreted. Similarity: Contains 5 EGF-like domains. Contains 1 WIF domain. SWISS: Q9Y5W5 Gene ID: 11197 Database links: Entrez Gene: 11197 Human Entrez Gene: 24117 Mouse Omim: 605186 Human SwissProt: Q9Y5W5 Human SwissProt: Q9WUA1 Mouse Unigene: 284122 Human Unigene: 32831 Mouse Unigene: 74256 Rat WIF-1(Wnt inhibitory factor-1)屬于Wnt分子胞外抑制劑中的sFRP(secreted Frizzled-related protein,sFRP) 家族,通過直接與Wnt分子結(jié)合, 而抑制Wnt信號(hào)通路活性. |
