| 產(chǎn)品編號 | bs-2345R |
| 英文名稱 | PDLIM1 Rabbit pAb |
| 中文名稱 | 細胞骨架蛋白C端PDLIM1抗體 |
| 別 名 | CLIM1; CLP-36; CLP36; HEL-S-112; hCLIM1; mClim1; PDLI1_HUMAN; PDLIM1; C-terminal LIM domain protein 1; Elfin; LIM domain protein CLP-36; PDLI1_MOUSE; Q3TZ17_MOUSE; PDLI1_RAT; |
| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學(xué) 染色質(zhì)和核信號 新陳代謝 表觀遺傳學(xué) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Chicken,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36 kDa |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PDLIM1: 251-329/329 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
PDLIM1 is a cytoskeletal protein that may act as an adapter that brings other proteins to the cytoskeleton. PDLIM1 is strongly expressed in the heart
and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung, and minimally expressed in liver, thymus, kidney, prostate and pancreas. It is not found in the brain, testis, ovary, and peripheral blood leukocytes. Function: Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. Subunit: Interacts with alpha-actinins 1, 2 and 4. Subcellular Location: Cytoplasm. Cytoplasm, cytoskeleton. Note=Associates with the actin stress fibers. Tissue Specificity: Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes. Similarity: Contains 1 LIM zinc-binding domain. Contains 1 PDZ (DHR) domain. SWISS: O00151 Gene ID: 9124 Database links: Entrez Gene: 9124 Human Entrez Gene: 54132 Mouse Omim: 605900 Human SwissProt: O00151 Human SwissProt: O70400 Mouse SwissProt: Q3TZ17 Mouse Unigene: 368525 Human Unigene: 5567 Mouse Unigene: 11170 Rat |
| 產(chǎn)品圖片 |
Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti- PDLIM1 (bs-2345R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD
|
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
