| 產(chǎn)品編號 | bs-1298R |
| 英文名稱 | Smac Rabbit pAb |
| 中文名稱 | 線粒體促凋亡蛋白抗體 |
| 別 名 | Smac/Diablo; 0610041G12Rik; DBLOH_HUMAN; DBOH; DBOH; diablo; Diablo homolog(Drosophila); Diablo homolog(Drosophila); Diablo homolog; Diablo homolog Drosophila; Diablo homolog mitochondrial; Diablo homolog mitochondrial precursor; DIABLO S; DIABLO S; DIABLOS; Direct IAP binding protein with low pI; Direct IAP-binding protein with low pI; FLJ10537; FLJ25049; mitochondrial; Mitochondrial Smac protein; Second Mitochondria Derived Activator of Caspase; Second mitochondria-derived activator of caspase; SMAC 3; smac; Smac protein; SMAC3. |
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Specific References (1) | bs-1298R has been referenced in 1 publications.
[IF=1.96] Liu, Bao-Heng, et al. "Smac/DIABLO regulates the apoptosis of hypertrophic scar fibroblasts."?International Journal of Molecular Medicine?(2013).
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| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 細(xì)胞凋亡 新陳代謝 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Pig,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 21 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Smac: 131-239/239 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Several alternatively spliced transcript variants that encode distinct isoforms have been described for this gene but the validity of some transcripts, and their predicted ORFs, has not been determined conclusively. The inhibitor of apoptosis (IAP) proteins regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes their inhibitory effect on caspases has been designated Smac/DIABLO. This is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates the cytochrome c/Apaf-1/caspase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues. Function: Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance. Subunit: Homodimer. Interacts with NGFRAP1/BEX3 (By similarity). Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2, XIAP/BIRC4, BIRC6/bruce and BIRC7/livin. Interacts with the monomeric and dimeric form of BIRC5/survivin. Subcellular Location: Mitochondrion. Note=Released into the cytosol when cells undergo apoptosis. Tissue Specificity: Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed. Post-translational modifications: Ubiquitinated by BIRC7/livin. DISEASE: Deafness, autosomal dominant, 64 (DFNA64) [MIM:614152]: A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Note=The disease is caused by mutations affecting the gene represented in this entry. SWISS: Q9NR28 Gene ID: 56616 Database links: Entrez Gene: 56616 Human Entrez Gene: 66593 Mouse Omim: 605219 Human SwissProt: Q9NR28 Human SwissProt: Q9JIQ3 Mouse Unigene: 169611 Human Unigene: 46716 Mouse Smac（second mitochondria-derived activator of caspase）又稱DIABLO（direct IAP binding protein），是一種線粒體蛋白，也是重要的促細(xì)胞凋亡蛋白之一，能夠促進(jìn)細(xì)胞凋亡，當(dāng)殺死不正常細(xì)胞的機(jī)制啟動后，線粒體釋放出Smac蛋白促使受損或不正常細(xì)胞凋亡的生化過程就會出現(xiàn)，Smac在誘導(dǎo)腫瘤細(xì)胞凋亡，增加腫瘤細(xì)胞的化療敏感性及增強(qiáng)免疫力等方面有一定的意義和應(yīng)用前景。Smac是第二個線粒體來源的胱氨酸酶激活劑，它通過拮抗或中和一種或幾種凋亡抑制蛋白（IAP）家族成員而發(fā)揮其促凋亡作用。 |
